Enzo Life Sciences公司的NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)和NUCLEAR-ID ® Red DNA Stain是細(xì)胞滲透性染料，適用于活細(xì)胞的細(xì)胞核染色。這系列染料為通過流式細(xì)胞術(shù)研究細(xì)胞周期進(jìn)程的誘導(dǎo)和抑制提供了一種簡便方法。該試劑在活細(xì)胞研究中的潛在應(yīng)用包括確定細(xì)胞DNA含量和細(xì)胞周期分布、檢測生長模式的變化、監(jiān)測細(xì)胞凋亡以及評估腫瘤細(xì)胞行為和抑制基因機(jī)制。

NUCLEAR-ID® Red DNA stain

產(chǎn)品特點(diǎn)

● 遠(yuǎn)紅色熒光特異性DNA染料

● 穩(wěn)定且純度高

● 可分析活細(xì)胞、可滲透細(xì)胞和固定細(xì)胞

● 無光漂白作用

● 無需RNase處理

● 與GFP和FITC兼容

● 不需要紫外線激光源激發(fā)

● 經(jīng)驗(yàn)證適用于各種細(xì)胞密度

● 快速且易于使用

實(shí)例分析

在37°C下用終濃度為4000x或2000x的NUCLEAR-ID ® Red DNA Stain或同等µM濃度的競爭品牌染料對細(xì)胞進(jìn)行染色，并在染色后輕輕洗滌。在15分鐘和24小時(shí)對細(xì)胞成像，結(jié)果顯示NUCLEAR-ID ® Red DNA Stain在更低的濃度就能顯現(xiàn)dsDNA。

產(chǎn)品詳情：

部分產(chǎn)品文獻(xiàn)引用

1. CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)

2. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)

3. Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)

4. DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)

5. Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)

6. Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)

7. Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)

NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)

產(chǎn)品特點(diǎn)

● 藍(lán)色熒光特異性DNA染料

● 穩(wěn)定且純度高

● 可分析活細(xì)胞、可滲透細(xì)胞和固定細(xì)胞

● 無光漂白作用

● 無需RNase處理

● 與GFP和紅色染料兼容

● 經(jīng)驗(yàn)證適用于各種細(xì)胞密度

● 快速易用

產(chǎn)品詳情：

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