CpG ODN, type B (murine) - TLR9-based adjuvant

CpG ODN 1826 is a synthetic immunostimulatory oligonucleotide (ODN) that contains unmethylated CpG dinucleotides.

ODN 1826 is a class B CpG ODN specific for mouse Toll-like receptor 9 (TLR9). It strongly activates B cells, weakly stimulates IFN-α secretion in pDCs and generates Th1-dominant immune responses.

ODN 1826 VacciGrade™ is prepared under strict aseptic conditions. It is guaranteed sterile and has been thoroughly tested to confirm the absence of endotoxins. ODN 1826 VacciGrade™ is suitable for preclinical studies.

ODN 1826 VacciGrade™ is for research use only; it is not for use in humans.

Specifications

Specificity: Th1 response

Working concentration: 20 - 50 μg/mouse

Quality: Sterile, Endotoxin level

Solubility: 2 mg/ml in physiological water

Quality control:
ODN 1826 VacciGrade™ is a preclinical grade preparation of the CpG ODN 1826. It is prepared under strict aseptic conditions.
It is tested for sterility and the presence of endotoxins.
ODN 1826 VacciGrade™ is guaranteed sterile and its endotoxin level is ODN1826 sequence: 5’-tccatgacgttcctgacgtt-3’ (20 mer)

Contents

ODN 1826 VacciGrade™ is provided lyophilized width:

• 1 mg (157.1 nmol) of sterile lyophilized ODN 1826 VacciGrade™

• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

 ODN 1826 VacciGrade™ is shipped at room temperature

 Upon receipt it should be stored at -20°C for up to 1 year.

Upon resuspension, prepare aliquots of ODN 1826 VacciGrade™ and store at -20°C.

Product is stable 6 months at -20°C when properly stored.

Avoid repeated freeze-thaw cycles.

Details

Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs), such as ODN 1826, have been extensively studied as adjuvants [1].

These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA [2].

CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses  [3].

Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses  [1].

Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities  [4-5]. Furthermore, CpG ODNs activate TLR9 in a species-specific manner  [6].

ODN 1826 is a type B CpG ODN specific for mouse TLR9. Type B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides and can strongly activate B cells  [4].

ODN 1826 has been tested as an adjuvant in numerous animal models  [7-9].

Research in mice demonstrated that ODN 1826 administration can induce the activation of antigen presenting cells8 and type I IFN anti-viral activity [8-9], indicative of a Th1 immune response.
 

1. Steinhagen F. et al., 2011. TLR-based immune adjuvants. Vaccine 29(17):3341-55.
2. Hemmi H. et al., 2000. A Toll-like receptor recognizes bacterial DNA. Nature 408:740-5.
3. Coffman RL. et al., 2010. Vaccine adjuvants: Putting innate immunity to work. Immunity 33(4):492-503.
4. Krug A. et al., 2001. Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur J Immunol, 31(7): 2154-63.
5. Marshall JD. et al., 2005. Superior activity of the type C class of ISS in vitro and in vivo across multiple species. DNA Cell Biol. 24(2):63-72.
6. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. PNAS, 98(16):9237-42.
7. Xiong Z. et al., 2008. Effective CpG immunotherapy of breast carcinoma prevents but fails to eradicate established brain metastasis. Clin Cancer Res 14: 5484-93.
8. Longhi MP. et al., 2009. Dendritic cells require a systemic type I interferon response to mature and induce CD4+Th1 immunity with poly IC as adjuvant. J Exp Med 206: 1589-602.
9. Ank MB. et al., 2008. An important role for type III interferon (IFN-lambda/IL-28) in TLR-induced antiviral activity. J Immunol 180: 2474-85.

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